1. Start -> Run -> Notepad.

2. Paste the following:

(for autoplay on):

Windows Registry Editor Version 5.00

[HKEY_CURRENT_USER\Software\Microsoft\Windows\CurrentVersion\Policies\Explorer]
"NoDriveTypeAutoRun"=dword:00000091
"NoSaveSettings"=dword:00000000

(save as autoplayon.reg)

(for autoplay off):

Windows Registry Editor Version 5.00

[HKEY_CURRENT_USER\Software\Microsoft\Windows\CurrentVersion\Policies\Explorer]
"NoDriveTypeAutoRun"=dword:00000095
"NoSaveSettings"=dword:00000000

(save as autplayoff.reg)